The MSF is located in A411 and is staffed from 9:30 to 5:30 daily and facility houses the following equipment: One Thermo MAT-95 XP magnetic sector mass spectrometer (EI and CI sources available, staff use only), two Waters LCT Classic electrospray/time-of-flight LC-MS systems (available for walk-up use), one Bruker Biflex III MALDI-TOF instrument (available for walk-up use), one Agilent 6890/5973 GC-MS (available for walk-up use), and one soon to be installed Agilent 1200/6310 ESI-quadrupole LC-MS system (available for walk-up use). See Dr. Jonathan Karty (facility manager) or Angela Hansen (Senior Mass Spectroscopist) for more information or to schedule training. Rates for IU faculty research groups can be found on the Instrumentation page of the MSF website.

Services provided:
- Walk-up electrospray ionization (ESI) and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry for both small molecules and biological samples
- Training for the IU community in the theory and practice of modern mass spectrometry
- High resolution, high accuracy (±5 parts per million, ppm) mass spectrometry of small molecules by electron impact, chemical ionization, and electrospray ionization
- High mass resolution, high mass accuracy gas chromatographic mass spectrometry
- Low mass resolution gas chromatographic mass spectrometry with both electron impact and chemical ionization sources
- Capillary-scale (minimum column i.d. = 0.3 mm) high performance liquid chromatographic mass spectrometry (HPLC-MS) with simultaneous detection of up to two UV-VIS wavelengths
- Analysis of air and water sensitive compounds by electron impact, chemical ionization, fast atom bombardment, electrospray ionization, and matrix assisted laser desorption/ionization

The Indiana University Molecular Structure Center laboratory has a full complement of single crystal and powder diffraction equipment used to characterize crystalline materials using the techniques of X-ray crystallography. Researchers in the laboratory can determine the three-dimensional structure of nearly any material that can be crystallized.

The results of a crystallographic study is a set of atomic coordinates which locate the atoms of a molecule in the "unit cell" of the crystal. This information can then be used to generate images of the molecule and to determine distances and angles in the molecule. In addition, the data allows one to examine the packing of the molecules in the crystal - information which can often lead to understanding the properties of the material.

IUMSC Server allows rapid access to the data that are generated in the IUMSC. Nearly all of the materials studied have been synthesized or isolated by researchers from other laboratories, usually within the Indiana University system, but often from laboratories throughout the world. This server supports these researchers to access to the data as soon as the study is complete.

The NMR Facility provides 5 high resolution NMR spectrometers for a variety of solution-state experiments. All spectrometers are manufactured by Varian Associates and are controlled by Sun workstations or RedHat Linux PCs. Two Windows PCs, three Sun Data Stations, and a RedHat Linux PC are available for post-acquisition data processing and analysis.

Inside the NMR facility is a 500 MHz Varian Inova NMR Spectrometer designed for long-term experiments at low concentrations, such as multidimensional experiments using biochemical molecules. It's predominantly used by chemists who need long-term, high resolution, or high sensitivity acquisitions.

Also within the NMR facility is a 400 MHz Varian Inova NMR Spectrometer designed for experiments of all NMR-active nuclei at various temperatures. It includes a Four Nucleus+ probe which can observe 1H, 19F, 31P, or 13C without assistance from the facility staff, and can be tuned to observe other nuclei. It is also capable of decoupling two nuclei (1H, 19F, 31P, or 13C) while observing a third nucleus (1H, 19F, 31P, or 13C).

The NMR Facility is equipped with several Data Stations for pre-acquisition experiment set-up and post-acquisition data processing, analysis, and interpretation. There is also a Wet Lab which can be used for routine chemistry related to NMR samples and is available upon request.

The Electron Paramagnetic Resonance Room provides support to research projects of the chemistry department as well as other departments in chemistry, physics, material science, and biology. Electron Paramagnetic Resonance is a spectroscopic technique for studying molecules that contain unpaired electrons, typically transition-metal ions and organic radicals, yielding structural and dynamic information. The EPR room is equipped with a Bruker EMX X-band EPR spectrometer with liquid-helium low temperature (down to 4K) accessories. Frank Gao is currently in charge of the spectrometer, with Professor Kenneth Caulton as the faculty adviser.

Over the past several years there has been a rapid increase on the IUB campus of research into the structures, stabilities and interactions of biomolecules. The Physical Biochemistry Instrumentation Facility was established to facilitate and encourage these research endeavors and to provide a centralized resource for training and education in modern physical biochemistry. Located in the new, state-of-the-art Simon Hall, it houses state-of-the-art biophysical instrumentation that is available for use by faculty and students.

Available Equipment:

• Perkin-Elmer LS50B Luminescence Spectrometer
• Beckman Coulter Optima XL-I Analytical Ultracentrifuge
• Microcal VP-ITC Isothermal Titration Calorimeter
• Amersham Biosciences Typhoon 9210 Variable Mode Imager
• Jasco J-715 Circular Dichroism Spectropolarimeter
• Varian Cary 100 Bio UV / Visible Spectrometer
• Malvern Instruments Zetasizer Nano-S Dynamic Light Scatterer
• BIACORE 3000
• Packard 1600TR Liquid Scintillation Counter